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Live cells were then washed with phosphate-buffered saline 1, incubated for 30 min with 5 M JC-1 (37 C, 5% CO 2 ), washed with PBS 1, trypsinized, and recovered in a final volume of 300 L of PBS

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Frequent temperature fluctuationscaused by opening and closing the fridge doorcan accelerate the breakdown of the peptide

For AsA, the supernatant was diluted by 250 mM phosphate buffer (pH 7.4) and mixed with 0.5% N-ethyl maleimide (NEM)

Non-ablative fractional lasers : Stimulate skin remodelling and may assist with diffuse pigmentation improvement over a series of treatments